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雙通道實(shí)時(shí)定量PCR 儀

【介紹】

DNA Engine Option 2 是一款雙波長(zhǎng)實(shí)時(shí)PCR 儀。96 個(gè)藍(lán)－綠 LED 順序激發(fā)樣品，通過二個(gè)光電二極管來檢測(cè)。兩個(gè)激發(fā)波長(zhǎng)范圍：一為523–543nm（適用于FAM、SYBR Green I 等），還有一個(gè)是 540–700nm（適用于普遍使用的HEX、TEMRA、TET、VIC 等熒光素）。

DNA Engine Option 2 既可以支持普通的單重PCR，也可以支持多重 PCR。多重 PCR 除了可以節(jié)省試劑和時(shí)間外，還可以使內(nèi)對(duì)照的使用成為可能，從而對(duì)樣品PCR 效率有更進(jìn)一步的掌控。此外，多重PCR 也使基因表達(dá)分析更為簡(jiǎn)便。其具體性能如下：

一次96 孔熒光檢測(cè)時(shí)間在3 秒之內(nèi)，最大程度減少了熒光交叉

完美的溫度控制性能和梯度PCR 功能

Opticon Monitor 分析軟件可以非常簡(jiǎn)單地設(shè)置RT–PCR、多重PCR 等反應(yīng)，以及DNA 定量、基因表達(dá)、等位基因等功能分析

LED 陣列與反應(yīng)板一一對(duì)應(yīng)，保證激發(fā)光準(zhǔn)確進(jìn)入目標(biāo)管。發(fā)射光通過光電倍增管（PMTs）系統(tǒng)進(jìn)行信號(hào)放大。

【技術(shù)指標(biāo)】

Base thermal cycler	DNA Engine
Sample capacity	96-well PCR plate (low-profile) or 12 x 0.2 ml 8-tube strips (low-profile)
Sample volume	10–100 µl (20 µl recommended)
Dimensions (W x D x H)	34 x 47 x 60 cm
Weight	29 kg
Fluorescence excitation range	470–505 nm
Fluorescence detection range	Channel 1: 523–543 nm Channel 2: 540–700 nm
Linear dynamic range of starting copy number	Up to 10 orders of magnitude
Detection limit of template starting copy number	Down to single initial template copy detection
Gradient Specifications
Gradient range	30–105°C
Temperature differential range	1–24°C
Gradient accuracy	±0.3°C of target at end columns within 30 sec of arrival at programmed target, NIST–traceable
Temperature uniformity	±0.4°C, in column, well-to-well, within 10 sec of target attainment
Gradient calculator accuracy	±0.4°C of actual column temperature (NIST–traceable)
Computer System Requirements (Computer NOT Included With System)
Model	Desktop tower computer
PCI card slot dimensions (L x H)	Full-size card, 280 mm (11 in) x 106 mm (4.2 in) or short card, 174 mm (6.9 in) x 106 mm (4.2 in)
Operating system	Windows XP Professional
Storage capacity	40 GB hard drive
Memory	512 MB RAM
Port	Communication (COM)
Supplied With System
Data acquisition board	National Instruments PCI-6036E, 200 x 10³ samples/sec
Note: Specifications subject to change without notice.